BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3 ). Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF.
Recombinant Human Brain-Derived Neurotrophic Factor produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26.984kD.
Endotoxin: <0.1EU/ug (LAL)
AA Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Biological Activity:
BDNF is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent induction of C6 cell proliferation is 1.3-2ug/ml.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O. Reconstituted product is stable for 6 months at -20 degrees C. For long term storage add 0.1% HSA or BSA, aliquot, and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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