Brain Natriuretic Peptide, Pro, NT, 46-61 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa "signal" peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.\n\nCellular Localization: Secreted\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

B2702-03W

Size

200ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

PBS [pH7.4] containing 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

Pfister R et al. Use of NT-proBNP in routine testing and comparison to BNP. Eur J Heart Fail 6:289-93 (2004). <PUBMED:14987578>Hall C Essential biochemistry and physiology of (NT-pro)BNP. Eur J Heart Fail 6:257-60 (2004). <PUBMED:14987573>Hobbs FD et al. Reliability of N-terminal pro-brain natriuretic peptide assay in diagnosis of heart failure: cohort study in representative and high risk community populations. BMJ 324:1498 (2002). <PUBMED:12077039>Mair J et al. The impact of cardiac natriuretic peptide determination on the diagnosis and management of heart failure. Clin Chem Lab Med 39:571-88 (2001). <PUBMED:11522102>

Additional Info

Species Crossreactivity: Crossreacts with Human.