Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12kD BNP precursor gives rise to N-terminal proBNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa103-134) promotes vasodilation and fluid and sodium excretion.
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1ug/ml. The detection limit is ~20ng/well for recombinant human BNP.
Immunohistochemistry: 25ug/ml detects proBNP in paraffin-embedded normal human heart tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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