BSRP-A (Brain-specific receptor-like protein A; also SEZ6L2) is a 170kD member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, and likely regulates PKC alpha activation. Mature mouse BSRP-A is an 890aa type I transmembrane protein. It contains an 824aa extracellular domain (ECD) (aa21-844) and a 43aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for BSRP-A. One shows a premature truncation after Arg403, a second shows a deletion of aa71-130, and a third likely represents the full-length form that contains a 13aa
insertion after Val830. Over aa28-837, mouse BSRP-A is 96% and 99% aa identical to human and rat BSRP-A, respectively.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA (Direct): 0.5-1ug/ml Detection limit for recombinant mouse BSRP-A is ~1ng/well.
Western Blot: 2ug/ml cell or tissue extracts
Immunohistochemistry: 25ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Myeloma cells with B cells from Balb/c mice.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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