The breast cancer tumor-suppressor gene, BRCA1, encodes a protein with a BRCT domain, a motif that is found in many proteins that are implicated in DNA damage response and in genome stability. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. BRCA1 functions in the nuclear processes of transcription, chromatin remodeling and silencing, and in various DNA repair mechanisms. It also has ubiquitin ligase activity. It regulates key effectors that control the G2/M checkpoint and is therefore involved in regulating the onset of mitosis. It is a 1,863-residue protein of unknown structure and has a few identifiable features. The C-terminus of BRCA1 contains two 95-residue BRCT domains. Mutations in the tumor-suppressor gene BRCA1 predispose individuals to breast, ovarian and breast-ovarian cancer. Many alternatively spliced transcript variants have been described for this gene with only some have had their full-length natures identified.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:15-1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Ovarian carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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