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BRD2 (Bromodomain-containing Protein 2, O27.1.1, Really Interesting New Gene 3 Protein, KIAA9001, RING3)

BRD2 (Bromodomain-containing Protein 2, O27.1.1, Really Interesting New Gene 3 Protein, KIAA9001, RING3)

Cat no: B2735-01E

Supplier: United States Biological
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Brd2 is a highly conserved member of the BET subfamily of bromodomain proteins that contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal (ET) domain (1). In addition to its involvement in guiding the expression of cell cycle genes through its binding to multiple E2Fs (2), Brd2 has been shown to be associated with several regulators of transcription, including TFIID and Swi/Snf complexes (3,4). First identified as a nuclear serine/threonine kinase (5), Brd2, like other bromodomain proteins, is thought to function in mammalian development by regulating chromatin structure and transcription (6). Brd2 has been shown to bind to histone H4 via acetylated Lys12, a substrate of several histone acetyltransferase transcriptional coactivators (7). In mouse, Brd2 has the highest levels of expression during embryogenesis and in the adult testis, ovaries, and brain (8,9,10). Brd2-deficient mouse embryos exhibit delayed development and eventual death due to neural tube closure defects (11). Mutations in the promoter of the Brd2 gene have been associated with increased susceptibility to juvenile myoclonic epilepsy (JME) (12). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2735-01E
Reactivities: Human, Mouse, Non-Human Primate
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Florence, B. and Faller, D.V. (2001) Front Biosci 6, D1008-18. 2.Denis, G.V. et al. (2000) Cell Growth Differ 11, 417-24. 3.Crowley, T.E. et al. (2002) Mol Endocrinol 16, 1727-37. 4.Denis, G.V. et al. (2006) J Proteome Res 5, 502-11. 5.Denis, G.V. et al. (2000) Cell Growth Differ 11, 417-24. 6.Gyuris, A. et al. (2009) Biochim Biophys Acta 1789, 413-21. 7.Kanno, T. et al. (2004) Mol Cell 13, 33-43. 8.Shang, E. et al. (2004) Gene Expr Patterns 4, 513-9. 9.Trousdale, R.K. and Wolgemuth, D.J. (2004) Mol Reprod Dev 68, 261-8. 10.Crowley, T.E. et al. (2002) Mol Endocrinol 16, 1727-37. 11.Gyuris, A. et al. (2009) Biochim Biophys Acta 1789, 413-21. 12.Pal, D.K. et al. (2003) Am J Hum Genet 73, 261-70.
Additional info: Recognizes endogenous levels of total human Brd2. Species Crossreactivity: Monkey and mouse.

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