Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa 52-139) and a C-terminal EGF-like (aa 646-682) and C-type lectin domain (aa 695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa 649-911 that serves as a GPI-anchor motif. Over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inibition. Other applications not tested. Recommended Dilutions: Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Brevican is ~1ng/well. Western Blot: 1-2ug/ml. The detection limit for recombinant human Brevican is ~10ng/lane under non-reducing conditions. Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium. Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve 100% blocking in the assay. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.