Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican contains an N-terminal V-type Ig-like domain (aa52-139), a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Multiple isoforms exist where there is an alternate start site at Met228 and a 23aa substitution for aa 649-911 that serves as a GPI-anchor motif. over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican.
Applications:
Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunocytochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Brevican (aa23-911).
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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