Home  >  Products  >  BRF2 (BRFU, FLJ11052, RNA Polymerase III Transcription Initiation Factor BRF2, TFIIIB50, Transcription Factor IIB-related Factor TFIIIB50)
BRF2 (BRFU, FLJ11052, RNA Polymerase III Transcription Initiation Factor BRF2, TFIIIB50, Transcription Factor IIB-related Factor TFIIIB50)

BRF2 (BRFU, FLJ11052, RNA Polymerase III Transcription Initiation Factor BRF2, TFIIIB50, Transcription Factor IIB-related Factor TFIIIB50)

Cat no: B2743-53

Supplier: United States Biological
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BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. Cellular Localization: Nuclear Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000. Western Blot: 1-3ug/ml. Predicted molecular weight 48kD. Detects a band of ~ 50kD Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B2743-53
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Schramm L et al. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. Genes Dev 14:2650-63 (2000).
Additional info: Species Crossreactivity: Crossreacts with Human.

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