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Brilliant Violet 421(TM) anti-human TNF-alpha

Brilliant Violet 421(TM) anti-human TNF-alpha

Cat no: 502931


Supplier: BioLegend
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TNF-alpha is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-alpha. Monomeric human TNF-alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Catalogue number: 502931
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry
Size: 25 tests
Clone: MAb11
Gene: 7124
Dilutions: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ?5 microl per million cells or 5 microl per 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421(TM) excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421(TM) is a trademark of Sirigen Group Ltd.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Antigen: E. coli-expressed, recombinant human TNF-alpha
Isotype: Mouse IgG1, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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