Brk (45-50kD) is 80% identity to Sik (Src-related intestinal kinase) and exhibits the features of a novel non-receptor tyrosine kinase, including amino terminal SH3 and SH2 domains. Low levels of Brk transcripts have been identified in some human breast tumor cell lines but not in normal breast tissue.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 2.5-5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
T47D cells. Breast carcinoma
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.