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Bromodeoxyuridine (BrdU)

Cat no: B2850

Bromodeoxyuridine (BrdU)

5-Bromo-2-deoxyuridine (BrdU) is an analog of thymidine, and can be incorporated into the newly synthesized DNA of replicating cells (S-phase cells). BrdU is a commonly used in the detection of proliferating cells in living tissues. It works by substituting for thymidine during DNA replication and incorporating itself into the newly synthesized DNA (during S-phase). Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Because BrdU can replace thymidine during the S phase of cell division, it can cause mutations. Bromodeoxyuridine (BrdU) is also used to prepare dNTP-BrdU solution for UV cross-linking of proteins to nucleic acids and to prepare complete MEM-10-Brdu or MEM-20-Brdu for vaccinia virus stock cultures. \n\nSolubility: Souble in 0.1N NaOH and DMF, sparingly soluble in water, slightly soluble in ethanol and acetone, practically insoluble in ether\n\nRatio of Absorbances (pH2):\n0.47-0.53 (A250/A260)\n1.87-1.93 (A280/A260)\n1.45-1.55 (A290/A260)\n\nSpecific Rotation (C=1%, H2O): +23.0 degrees C\n\nRNase: None Detected\n\nDNase: None Detected\n\nProtease: None Detected\n\nEndotoxin: (same/less than)0.5EU/mg

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SPECIFICATIONS

Catalog Number

B2850

Size

1g

Form

White to off white, crystalline powder

Purity

(same/more than)98%

References

US Biological application reference: Betancourt, J. et al., (2013) J. Comparative Neurology DOI:

Alternative Names

5-Bromo-2

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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