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Bromodeoxyuridine (BrdU)

Bromodeoxyuridine (BrdU)

Cat no: B2850-01E

Supplier: United States Biological
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BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating DNA. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen, paraffin): 1:5-1:10 Dilute in 0.15M PBS, 1% BSA, 1% sodium-azide. Requires protease digestion of paraffin sections. Immunocytochemistry: Pulse labeling of dividing cells will allow the immunocytochemical identification of S-phase cells. Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: Bromodeoxyuridine labeled cells. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2850-01E
Hosts: Mouse
Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Size: 1ml
Form: Supplied as a liquid, 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Ascites
References: 1. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 371-374. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 1343-1345 2. Schutte, B., et al., 1987, Cytometry 8, 372-376.
Additional info: Recognizes DNA in which BrdU has been incorporated. Reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. Recognizes BrdU in denaturated (single stranded) DNA. 100% crossreactive with Jodo-Deoxy-Uridine (IrdU).

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