Bromodeoxyuridine (BrdU)

Cat no: B2850-01K

Supplier: United States Biological

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Description
Additional Information

BrdU is a thymidine analog that, when added to replicateing cells, is incorporated into newly synthesized DNA strands. Evaluating the uptake of BrdU by dividing cells can provide a reliable estimation of cellular prolieration rates. BrdU immunohistochemistry may prove useful in determining proliferative rates for the evaluation of tumors. Positive Control: Bromodeoxyuridine labeled cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry (Fr) (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	B2850-01K
Reactivities:	Human
Hosts:	Mouse
Applications:	Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Size:	100ul
Form:	PBS with 1% BSA containing 0.09% sodium azide
P type:	Mab
Isotype:	IgG1
Purity:	Ascites
References:	Dolbeare F. Histochem J 28: 531-75, 1996 Heatley MK Histol Histopathol 13: 249-54, 1998 Hirano T, et al. Laryngoscope 101: 130-133, 1999 Lacy ER, et al. Gastroenterology 100: 259-262, 1991 Miller MA, et al. J Histochem Cytochem 39(4): 407-412, 1991 Ohyama S, et al. Cancer 69: 314-321, 1992 Ormerod Md Methods Mol Biol 75: 357-65, 1997 Shutte B, et al. Cytometry 8: 372-376, 1987 Shutte B, et al. J Histochem Cytochem 35:1343-1345, 1987 Shutte B, et al. J Histochem Cytochem 35:371-374, 1987 Welberg JW, et al. J Clin Pathol 43: 453-456, 1990 Nadji M, Morales AR, Immunoperoxidase, part 1: the techniques and its pitfalls, Lab Med 14:767-770, 1983
Additional info:	The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies. Species Crossreactivity: Crossreacts with human.

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Bromodeoxyuridine (BrdU)

Cat no: B2850-01K

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