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Bromodeoxyuridine (BrdU)

Cat no: B2850-07D


Supplier: United States Biological
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BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: (same/less than)2ug/10e5 cells in 100ul Immunohistochemistry: Formalin fixed paraffin embedded Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2850-07D
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG1,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Magaud, J-P., et al. 1989. J. Histochem. Cytochem. 37:1517. 2. Tomihari, M., et al. 2010. Cancer Res. 70:5778. 3. Gratzner, H.G. 1982. Science 218:474. 4. Dolbeare, F., et al 1983. Proc. Natl. Acad. Sci. USA 80:5573. 5. Dolbeare, F., et al. 1985. Cytometry 6:521.
Additional info: Recognizes human BrdU.

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