Bromodeoxyuridine (BrdU)

Cat no: B2850-10G

Supplier: United States Biological

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Description
Additional Information

Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	B2850-10G
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry
Size:	200ug
Form:	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein G affinity chromatography.
Alternative names:	BUdr
References:	1. Magaud, J.P. et al. (1989) Double Immunocytochemical labelling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem.37:1517-1527.
Additional info:	Recognizes the thymidine analog bromodoxyuridine (BrdU).

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Bromodeoxyuridine (BrdU)

Cat no: B2850-10G

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