Home  >  Products  >  Bromodeoxyuridine (BrdU)
Bromodeoxyuridine (BrdU)

Bromodeoxyuridine (BrdU)

Cat no: B2850-24C

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
5-bromodeoxyuridine (BrdU) is an analogue of thymi- dine. When incubated with tissue, proliferating cells incorporate BrdU into the DNA during S phase and this provides an accurate means of determining cell prolifer- ation. This antibody detects incorporated BrdU in cells or tissues. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:200-1:400 for 1 hr. at RT Optimal dilutions to be determined by the researcher. Positive Control: Cells or tissues with incorporated Brdu, nuclear staining pattern Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B2850-24C
Hosts: Mouse
Applications: Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
P type: Mab
Isotype: IgG1
Purity: Supernatant
Alternative names: 5-Bromo-2'-deoxyuridine
References: 1.Acta Histochemica, Supplement. Band XXXVI. 353-359 (1988). 2.Raza A, Ucar K and Preisler H D. Double labelling and in vitro versus in vivo incorporation of bromodeoxyuridine in patients with acute nonlymphatic leukaemia. Cytometry. 6:633-640 (1985). 3.Raza A, Preisler H D, Mayers G L, et al.. Rapid enumeration of S-phase cells by means of monoclonal antibodies. New England Journal of Medicine. 310(15):991 (1984). 4.Gratzner H G. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science. 218:474-475 (1982).
Additional info: Recognizes Bromodeoxyuridine. Species Crossreactivity: Any species with incorporated BrdU.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave