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Bromodeoxyuridine (BrdU) (Biotin)

Bromodeoxyuridine (BrdU) (Biotin)

Cat no: B2850-01U

Supplier: United States Biological
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BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Suitable for use in Flow cytometry. Other applications have not been tested. Recommended Dilutions: Flow cytometry: Neat. Use 20ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2850-01U
Hosts: Rat
Applications: Flow Cytometry
Conjugates: Biotin
Size: 100ug
Form: Suppplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Alternative names: 5-Bromodeoxyuridine
References: 1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369.
Additional info: Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not cross react with thymidine but does react weakly with chlorodeoxyuridine.

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