Home  >  Products  >  BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12)
BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12)

BRSK2, NT (BR Serine/threonine Kinase 2, Serine/Threonine-protein Kinase 29, Serine/Threonine-protein Kinase SAD-A, C11orf7, PEN11B, STK29, HUSSY-12)

Cat no: B2673-76D

Supplier: United States Biological
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Brain-specific kinase 1 (BRSK1; SAD-B) and Brain-specific kinase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of g-tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 in vitro diminishes axonal growth (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2673-76D
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Bright, N.J. et al. (2008) J Biol Chem 283, 14946-54. 2.Inoue, E. et al. (2006) Neuron 50, 261-75. 3.Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92. 4.Kishi, M. et al. (2005) Science 307, 929-32. 5.Choi, Y.J. et al. (2008) Genes Dev 22, 2485-95.
Additional info: Recognizes endogenous levels of total human BRSK2. Species Crossreactivity: Mouse and rat.

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