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Brucella abortus

Cat no: B2900-04J


Supplier: United States Biological
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Brucella abortus is a Gram-negative facultative intracellular bacterium which causes brucellosis; a chronic disease which results in abortions in cattle and undulant fever, endocarditis, and arthritis in humans. In humans, the disease is transmitted either through contaminated or untreated milk and its derivatives, or through direct contact with infected animals. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:20-1:200 Western Blot: 1:10-1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B2900-04J
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.01M PBS, pH 7.2, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein A affinity chromatography.
References: 1. Golding, B. et al. (2001) Immunity and protection against Brucella abortus. Microbes Infect. 3: 43-48.
Additional info: Recognizes the lipopolysaccharide (LPS) of Brucella abortus at ~10-30kD.

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