Bsp119I (AsuII)

Sequence:\n5'-T T^C G A A-3' \n3'-A A G C^T T-5'\n\nConcentration: 10u/ul \n\nSource: Bacillus species RFL119 \n\nStorage Buffer: \nSupplied as a liquid in10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.\n\nDiluent Buffer:\n10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.\n\nSupplied with: \nR1625: Restriction Enzyme Buffer A, 10X:\nSuplied as a liquid in 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA.\n\nEnzyme Properties\nCompatible Ends: \nBsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp11406I, Ssil, TaqI, XmiI \n\nMethylation Effects: \nDam, Dcm, EcoBI: Never overlaps - No effect.\nCpG: Completely overlaps - Blocked.\nEcoKI: May overlap - Effect not determined. \n\nStability during Prolonged Incubation: \nA minimum of 0.1units of Bsp119I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degrees C. \n\nThermal Inactivation: \nBsp119I is inactivated by incubation at 80 degrees C for 20min. \n\nDigestion of Agarose-embedded DNA: \nA minimum of 5units of Bsp119I is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. \n\nNumber of Recognition Sites in DNA:\nLambda: 7 \nPhiX174, M13mp18/19, pBR322, pUC18/19, pUC57, pTZ19R/U, pTZ57R, pBluescriptIIKS(+/-), pBluescriptIISK(+/-): 0\npACYC177: 1\npACYC184: 2\n\n

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