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BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)

BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD (BTAF1, KIAA0940, Mot1 Homolog (S. cerevisiae), TAF172, TAFII170, TAF(II)170)

Cat no: B3120-50

Supplier: United States Biological
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Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/ml Western Blot: 0.5-2ug/ml Immunocytochemistry: 2-50ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B3120-50
Reactivities: Human, Mouse, Rat
Hosts: Mouse
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes human BTAF1 RNA Polymerase II, B-TFIID Transcription Factor-Associated, 170kD. Species Crossreactivity: Mouse and rat.

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