BU-1A (Bu1a, B6.1)

Cat no: B2788-50D

Supplier: United States Biological

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Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200 -1:1,000; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	B2788-50D
Reactivities:	Chicken/Bird
Hosts:	Mouse
Applications:	Flow Cytometry, Immunohistochemistry
Size:	250ug
Form:	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein G affinity chromatography.
References:	1. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. Eur. J. Immunol. 19: 239-243.
Additional info:	Recognizes the 'a' allele of the polymorphic cell surface antigen Bu-1.

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BU-1A (Bu1a, B6.1)

Cat no: B2788-50D

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