BU-1A (Bu1a, B6.1) (FITC)

Cat no: B2788-50D1

Supplier: United States Biological

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Bu-1a is a 70kD alloantigen expressed on B cells and a subset of macrophages. Reports suggest that Bu-1 is expressed on B cell precursors at early stages of development. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:20-1:80; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Catalogue number:	B2788-50D1
Reactivities:	Chicken/Bird
Hosts:	Mouse
Applications:	Flow Cytometry
Size:	100ug
Form:	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein G affinity chromatography.
References:	1. Houssaint, E. et al. (1989) Bu-1 antigen expression as a marker for B cell precursors in chicken embryos. Eur. J. Immunol. 19: 239-243.
Additional info:	Recognizes the 'a' allele of the polymorphic cell surface antigen Bu-1.

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BU-1A (Bu1a, B6.1) (FITC)

Cat no: B2788-50D1

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