Home  >  Products  >  BUB1 (Bub1, Bub-1, BUB1A, BublL, Budding Uninhibited by Benzimidazoles 1-like, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog (Yeast Homolog), hBUB1, Mitotic Checkpoint Serine/threonine Protein Kinase BUB1)

BUB1 (Bub1, Bub-1, BUB1A, BublL, Budding Uninhibited by Benzimidazoles 1-like, BUB1 Budding Uninhibited by Benzimidazoles 1 Homolog (Yeast Homolog), hBUB1, Mitotic Checkpoint Serine/threonine Protein Kinase BUB1)

Cat no: B2790-01C


Supplier: United States Biological
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Applications: Suitable for use in Immunofluorescence, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1:200 Western Blot: 1:500-1:2,000 Immunofluorescence: 1:500-1:2,000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: B2790-01C
Reactivities: Human
Hosts: Mouse
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Alternative names: Mitotic Spindle Checkpoint Kinase, Putative Serine/threonine Protein Kinase
References: 1. Morrow, C.J. et al. (2005) Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/CCdc20. J. Cell Sci. 118: 3639-3652, 2. Meraldi, P. and Sorger, P.K. (2005) A dual role for Bub1 in the spindle checkpoint and chromosome congression. EMBO J. 24: 1621-1633, 3. Vanoosthuyse, V. and Hardwick, K.G. (2005) Bub1 and the multilayered inhibition of Cdc20-APC/C in mitosis. Trends Cell Biol. 15: 231-233.
Additional info: Recognizes Bub1, a protein kinase involved in spindle checkpoint function during mitosis.

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