Bub3 (Budding uninhibited by benzimidazoles 3 homolog; also Mitotic checkpoint protein Bub3) is a 35-40kD member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase
transition, until microtubules are properly aligned with, and bound to, chromatid kinetochores. Human Bub3 is 328aa in length. It contains five WD40 repeats (aa5-43, 46-83, 86-124, 128- 163 and 223-262) plus a phosphorylation site at Ser211 and a Lys at #216 that may form an interchain crosslink with Gly. There are two splice variants that show two or four aa deletion over the four C-terminal amino acids. Full-length human and mouse Bub3 share 99% aa identity.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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