Home  >  Products  >  BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1 beta, Protein SSK1)
BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1 beta, Protein SSK1)

BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1 beta, Protein SSK1)

Cat no: B2790-42

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
The Mitotic Checkpoint Complex (MCC) , which contains Bub1, Bub1b, Bub3, Mad2 and Cdc20, controls chromosome segregation and monitors kinetochoremicrotubule interactions (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/ Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6) and is required for functional microtubule-kinetochore interactions (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: B2790-42
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: Budding Uninhibited by Benzimidazoles 1 Homolog beta (Yeast), EC=2.7.11.1
References: (1) Fukagawa, T. (2008) Front Biosci 13, 2705-13. (2) Chen, R.H. (2007) J Biomed Sci 14, 475-9. (3) Dai, W. et al. (2004) Cancer Res 64, 440-5. (4) Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85. (5) Baker, D.J. et al. (2004) Nat Genet 36, 744-9. (6) Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11. (7) Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.
Additional info: Recognizes endogenous levels of total Bub1b.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave