Butyrylcholinesterase, Recombinant, Mouse (BCHE)

Source:\nSynthetic peptide corresponding to recombinant mouse Butyrylcholinesterase (1-603; Q543J3) expressed with a C-terminal 6X-His tag in a murine myeloma cell line, NS0. \n\nMolecular Mass: \nThe purified, secreted rmBCHE has a N-terminal sequence, H28TEEDFIITT. The 582 amino acid residue rmBCHE predicts a molecular mass of 66kD. By SDS-PAGE under reducing conditions, \nthe protein migrates with a molecular mass of approximately 86kD. \n\nMW: 66kD \n\nActivity: \nMeasured by its ability to hydrolyze butyrylthiocholine. The specific activity, measured under the described conditions, is (same/more than) 75 nmol/min/ug. \n\nEndotoxin: (same/less than)1.0 EU/1ug (LAL)\n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

B9101

Size

10ug

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution of 25mM tris, 0.15 M NaCl, pH 7.5.\n

Purity

(same/more than) 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain.

References

1. Darvesh, S. et al., 2003, Nat. Rev. Neuroscience 4:131. 2. Diamant, S. et al., 2006, Proc. Natl. Acad. Sci. USA 103:8628. 3. Campbell, V. A. and Gowran, A. 2007, Br. J. Pharm. 152:655. 4. Das, U. N. 2007, Med Sci Monit. 13:RA214.