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C-C Motif Chemokine 22, Human, BioAssay(TM) ELISA Kit (CC Chemokine STCP-1, MDC (1-69), Macrophage-derived Chemokine, MDC, Small-inducible Cytokine A22, Stimulated T-cell Chemotactic Protein 1, CCL22, SCYA22)

Cat no: 138797

C-C Motif Chemokine 22, Human, BioAssay(TM) ELISA Kit (CC Chemokine STCP-1, MDC (1-69), Macrophage-derived Chemokine, MDC, Small-inducible Cytokine A22, Stimulated T-cell Chemotactic Protein 1, CCL22, SCYA22)

The human C-C motif chemokine 22 (CCL22) or Macrophage-derived chemokine (MDC) BioAssay(TM) Kit is a sandwich ELISA. The capture antibody is a monoclonal human CCL22 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known CCL22 concentration are added to these wells and allowed to complex with the bound CCL22 antibody. A biotinylated human CCL22 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CCL22 present in the samples. \n\nIntended Use:\nThe purpose of this kit is the in-vitro quantitative determination of human CCL22 in samples such as sera, plasma, tissue lysates and cell culture supernates. This BioAssay(TM) kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.\n\nSensitivity:\n<1pg/ml\n\nRange:\n31.2pg/ml-2000pg/ml; 15.6pg/ml-1000pg/ml\n\nSpecificity:\nHuman C-C motif chemokine 22 (CCL22)\n\nKit components:\nPre-coated strip plates, 2x96wells\nProtein standards\nDetection reagents\nSubstrate buffer\nPrecise instructions\n\nStorage and Stability:\nStore other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

138797

Size

2Plates

Applications

ELISA

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