Home  >  Products  >  C-Cbl, active, Recombinant, Human, GST-Tag (E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, Signal transduction protein CBL, CBL2, RNF55)
C-Cbl, active, Recombinant, Human, GST-Tag (E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, Signal transduction protein CBL, CBL2, RNF55)

C-Cbl, active, Recombinant, Human, GST-Tag (E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55, Signal transduction protein CBL, CBL2, RNF55)

Cat no: 029430

Supplier: United States Biological
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Intracellular signals mediated by receptor tyrosine kinases play a pivotal role in morphogenesis, cell fate determination and pathogenesis. c-Cbl is a ubiquitin ligase (E3) that facilitates the regulation of these pathways, attenuating signalling through ubiquitination and subsequent protein degradation. c-Cbl contains several functional domains, including a tyrosine kinase-binding domain that associates with a number of protein tyrosine kinases in a phosphorylation-dependent manner, a catalytic RING-finger domain that interacts with E2s and is essential for E3 activity and a C-terminal proline rich domain which mediates interactions with SH3 containing proteins. Mutations in the gene for c-Cbl have been implicated in a number of human cancers, particularly acute myeloid leukaemia. Source: Recombinant corresponding to full length human c-Cbl, fused to GST-tag at N-terminal, expressed by baculovirus in Sf21 insect cells. Molecular Weight: ~127kD Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 029430
Reactivities: Human
Size: 10ug
Form: Supplied as a liquid in 50mM Tris/HCl pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Purity: ~75% (SDS-PAGE, Coomassie blue staining)
References: 1. Thien C.B. and Langdon W.Y. c-Cbl and Cbl-b Ubiquitin Ligases: Substrate Diversity and the Negative Regulation of Signalling Responses. Biochem J. 391(Pt 2): 153-66 2005. 2. Caligiuri M. A. Novel c-CBL and CBL-b Ubiquitin Ligase Mutations in Human Acute Myeloid Leukemia. Blood, 110: 1022-1024, 2007. 3. Joazeiro C. A. P. et al., The Tyrosine Kinase Negative Regulator c-Cbl as a RING-Type, E2-Dependent Ubiquitin-Protein Ligase. Science, 286; 309-312, 1999. 4. Yokouchi M. et al., Src-catalyzed Phosphorylation of c-Cbl Leads to the Interdependent Ubiquitination of Both Proteins. J Biol Chem. 276: 35185-35193, 2001. 5. Rao N. et al., The Cbl family of ubiquitin ligases: critical negative regulators of tyrosine kinase signaling in the immune system. J Leukoc Biol. 71: 753-763, 2002.

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