The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPb, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPb regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPb is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32kD in rodents, ~43kD and 40kD in humans) and a truncated inhibitory form known as LIP (~20kD) which lacks the N-terminal transcription activation domain. C/EBP beta is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPb is also acetylated at Lys39 in the transcription activation domain, and mutation of Lys39 impairs its ability to activate transcription of multiple genes, including several involved in adipogenesis.
Applications:
Suitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:20
Western Blot: 1:2000
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
PC-12 cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
