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c-erbB-2 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, CD340, C-erbB-2, HER2, HER-2, HER-2/neu, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2)

Cat no: C0016-01E

c-erbB-2 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, CD340, C-erbB-2, HER2, HER-2, HER-2/neu, MLN 19, NEU, NEU proto-oncogene, NGL, p185erbB2, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2)

v-ErbB was identified as the viral counterpart of the EGF Receptor, and its relatives have adopted this nomenclature (or the HER nomenclature for human proteins). These proteins are activated by Heregulin, Neuregulin and Amphiregulin. ErbB-2/HER-2, also known as neu, is amplified in large numbers of highly aggressive breast cancers, but all the family members are capable of heterodimerization, and together activate downstream signaling pathways. ErbB-3 has a natural "substitution" at the codon which is an invariant Lys residue at the ATP-binding site of all protein kinases. It is therefore inactive as a kinase, and can only become phosphorylated by heterodimerization with another erbB-family member. All members of this family, when Tyr-phosphorylated, can nucleate the formation of signaling complexes through adapter proteins.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry: Paraffin\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \nA431 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C0016-01E

Size

100ug

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in PBS, 1mg/ml BSA, 10mM sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human c-erbB-2. Species Crossreactivity: mouse and rat.

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