c-erbB3, Recombinant, Human (HER 3, ErbB3)

ErbB3/Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 can induce specific antibody production in vivo, hence to inhibit tumor cell growth. ErbB-3 can be used to treat early, medium and advanced or post-operative breast cancer with over-expression of ErbB2. According to its mechanism of action, ErbB3 is classified as a therapeutic for cancer.\n\nTyrosine Kinase ErbB3 Human Recombinant (HER3) produced in E.Coli is a single, non-glycosylated polypeptide consisting of several epitopes of extracellular domain of human Erb-b3, and having a total molecular mass of approximately 12kD.\nThe ErbB3 is purified by proprietary chromatographic techniques.\n\nBiological Activity: \nRecombinant ErbB3 is fully biologically active when compared to standard. Measured by its ability to postpone tumor emerge time of spontaneous breast cancer in FVB/N transgenic mice and inhibit the development of tumor, effectively inhibit the growth of in situ transplanted breast cancer in FVB/N transgenic mice.\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. For long tern storage, add a carrier protein (0.1% BSA or HSA) and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0026-05

Size

5ug

Form

Supplied as a supension in 1mg aluminum hydroxide 10mM arginine, 10mM sodium chloride, 20mM sodium phosphate buffer and 5mM potassium phosphate. \nIt is recommended that sterile PBS containing 1mg aluminum hydroxide be added to the vial to prepare a stock solution.

Purity

(same/more than) 95% as determined by RP-HPLC and SDS-PAGE.

References

1. ErbB-3 mediates phosphoinositide 3-kinase activity in gefitinib-sensitive non-small cell lung cancer cell lines.Proc Natl Acad Sci U S A 2005 Mar 8;102(10):3788-93. 2. Expression of ErbB-3 and ErbB-4 protooncogene proteins in oral squamous cell carcinoma: a pilot study. Med Oral 2003 Nov-Dec;8(5):374-813. Inhibition of ErbB-2 and ErbB-3 expression by quercetin prevents transforming growth factor alpha (TGF-alpha)- and epidermal growth factor (EGF)-induced human PC-3 prostate cancer cell proliferation. Int J Oncol 2003 Sep;23(3):821-9 4. The deaf and the dumb: the biology of ErbB-2 and ErbB-3. Exp Cell Res 2003 Mar 10;284(1):54-65. 5. Selective formation of ErbB-2/ErbB-3 heterodimers depends on th e ErbB-3 affinity of epidermal growth factor-like ligands. J Biol Chem 2003 Apr 4;278(14):12055-63. 6. Oncogene 2002 Aug 15;21(36):5609-18