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c-Jun, phosphorylated (Thr93)

c-Jun, phosphorylated (Thr93)

Cat no: C5815-01R

Supplier: United States Biological
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c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91and 93 in response to anisomycin in macrophages and TNF- alpha or anisomycin in fibroblasts (5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Catalogue number: C5815-01R
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
P type: Mab
Isotype: IgG
Purity: Supernatant
Alternative names: AP1, Activator Protein 1, Enhancer Binding Protein AP1, JUN, JUN protein, JUNC, Jun Activation Domain Binding Protein, Jun oncogene, Proto oncogene cJun, Transcription Factor AP1, V jun avian sarcoma virus 17 oncogene homolog, p39, v-Jun Avian Sarcoma Virus 17 Oncogene Homolog
References: 1. Behrens A, et al. Nat Genet. 21(3):326-9, 1999 2. Eferl R, et al. Cell. 112(2):181-92, 2003 3. Weiss C., et al. EMBO J. 22(14): 3686-95, 2003 4. Papavassiliou, A.G., et al. EMBO J. 14(9): 2014-2019,1995 5. Morton S., et al. The EMBO Journal 22, 3
Additional info: Recognizes human c-Jun phosphorylated at Thr93. Species Crossreactivity: Mouse.

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