c-Met, phosphorylated (Tyr1230, Tyr1234, Tyr1235), Human (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR) BioAssay(TM) ELISA Kit

Sensitivity: \n<0.25units/ml. The analytical sensitivity of this assay is <0.25units/ml of c-Met [pYpYpY1230/1234/1235]. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times.\n\nSpecificity:\nRecognizes human c-Met which is phosphorylated at tyrosine 1230, tyrosine 1234, and tyrosine 1235. Cross-reactivity with other species has not been determined.\n\nSpecifications:\nRange: 1.56-100units/ml\nIncubation Time: 4 hours\nSimple: Pre-coated strip-well plates\nLiquid stable chromogen and stop reagents\nSample size: 10ul (diluted to a total volume of 100ul)\nEconomical: One 96-well plate, plus reagents for 96 determinations\nReagents are stable for multiple runs.\nSample type: Cell extracts/lysates, buffered solutions\nPrecision: Intra-assay: (same/less than)7.8%\nInter-assay: (same/less than)8.8%\n\nKit Components:\nM3007-11A Standard 2x1vial\nM3007-11B Standard Diluent Buffer1x25ml\nM3007-11C Microtiter Plate, 1x96well \nM3007-11D c-Met Pab 1x11ml\nM3007-11E IgG (HRP) (100x) 1x125ul\nM3007-11F HRP Diluent. 1x 25ml\nM3007-11G Wash Buffer Concentrate (25x) 1x100ml \nM3007-11H TMB 1x25ml \nM3007-11J Stop Solution; 1x25ml \n\nStorage and Stability:\nStore at 4 degrees C. Store M3007-11A powder at 4 degrees C liquid at -70 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3007-11

Size

1Kit

Applications

ELISA

References

1. Crostella, L., et al. (2001) Oncogene 20(28):3735-3745. 2. Fan, S., et al. (2001) . Mol. Cell. Biol. 21(19(49):5582-5589. 5. Guiton, M., et al. (1994) J. Biol. Chem. 269(48):30370-30377. 6. Longati, P., et al. (2001) Curr. Drug Targets 2(1):41-55. 7. Kang, J.Y, et al. (2003) Cancer Res. 63:1101-1105.8. Matsumoto, K. and T. Nakamura (2001) Kidney Int. 59(6):2023-2038. 9. Maulik, G., et al. (2002) Cytokine Growth Factor Rev. 13(1):41-59. 10. Park, M., et al. (1987) Proc. Nat