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c-myc (Myc, myc-1, Cellular myelocytomatosis oncogene, Myc2, Niard, Nird, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog) (AP)

c-myc (Myc, myc-1, Cellular myelocytomatosis oncogene, Myc2, Niard, Nird, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog) (AP)

Cat no: C0034-10D

Supplier: United States Biological
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The c-myc gene product, is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:500 -1:5000 Western Blot: 1:100 -1:500, detects a band of ~62kD in Colo 320HSR cell lysates. Immunohistochemistry (frozen and paraffin): Does not require protein digestion pre-treatment of paraffin sections prior to staining. Does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Optimal dilution determined by the researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of themyeloma cell line. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Protect from light.
Catalogue number: C0034-10D
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid PBS, pH 7.4, with 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purifed by Protein G immunoaffinity chromatography.
References: 1. Evan, G.I. et al. (1985) Mol. Cell. Biol. 5:3610-3616. 2. Spandidos, D.A. et al. (1987) Anticancer Res. 7:1299 -1304.
Additional info: Recognizes the 62kD c-myc gene.

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