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C-Peptide (Hyperproinsulinemia, Insulin Precursor, IRDN, Proinsulin)

C-Peptide (Hyperproinsulinemia, Insulin Precursor, IRDN, Proinsulin)

Cat no: C7905-01Q

Supplier: United States Biological
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C-peptide is a 31 amino acid peptide released when proinsulin is split into insulin and C-peptide. In rats, two isoforms of proinsulin exists, the difference is two amino acid residues in the C-peptide, termed C-peptide I and C-peptide II. Proinsulin is split when it is released from the pancreas into the blood in response to a rise in serum glucose levels. C-peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2+ concentration and subsequent stimulation of N+, K+-ATPase and endothelial nitric oxide synthase activities. C-peptide also functions in repair of the muscular layer of arteries. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7905-01Q
Reactivities: Mouse, Rat
Hosts: Mouse
Applications: ELISA
Size: 200ug
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes an epitope in the N-terminal region of rat C-peptide I and II with almost equal affinity. Species Crossreactivity: mouse. Reacts weakly with mouse C-peptide II. It does not recognize rat proinsulin.

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