C-Peptide I & II, CT (Hyperproinsulinemia, Insulin Precursor, Proinsulin)

C-Peptide is part of the molecule of Proinsulin, that consists of three parts: C-Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C-Peptide are produced. C-Peptide is released into the blood stream in equal amounts to insulin. A test of C-Peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Although C-Peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2++, K+ -ATPase and endothelial nitric oxide synthase activities. C-Peptide also functions in repair of the muscular layer of arteries. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice Recommended antibody pairs for immunoassay. Crossreactivity of the recommended pairs with native rat proinsulin is <0.1%. Capture MAb: C7905-20A, biotinylated, 0.4ug/well Detection MAb: C7905-19A, Eu-labeled, 0.4ug/well To detect Rat C-peptides I & II: Capture Detection C7905-20A C7905-19A C7905-19A C7905-20A C7905-19B C7905-20A C7905-19C C7905-20A To detect Rat C-peptide I: Capture: C7905-19D Detection: C7905-20A Calibration curve for one step rat C-peptide immunoassay.Streptavidin coated plates Incubation time: 30 min. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.