Recombinant hCRP is produced into culture medium by DNA recombinant procedure in E. coli as secretory protein. By expressing kil gene, the outer membrane of E. Coli becomes permeable, allowing Recombinant hCRP to be excreted without cell lysis. Recombinant hCRP has similar biological property in terms of Ca2+-binding ability to the native protein, and is purified by affinity chromatography. N-terminal amino acid sequence of Recombinant hCRP is the same as that of the native human CRP.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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