C-Reactive Protein, High Sensitivity, Human, BioAssay(TM) ELISA Kit (CRP)

Intended Use:\nThe United States Biological C-Reactive Protein, High Sensitivity, Human, BioAssay(TM) ELISA Kit is intended for the quantitative determination of C-Reactive Protein (CRP) in human serum. Enhanced sensitivity measurements of CRP can be useful for the detection and evaluation of infection, tissue injury, inflammatory disorders and associated diseases. \n \nIntroduction:\nC-Reactive Protein (CRP) was identified by Tilet and Francis (1930) in the plasma of patients with pneumonia, and was named for its ability to bind and precipitate the C-polysaccharide of pneumococcus. It is an alpha globulin with a molecular mass of ~110-140kD, and is composed of five identical subunits, which are noncovalently assembled as a cyclic pentamer. CRP is synthesized in the liver and is normally present as a trace constituent of serum or plasma at levels less than 0.3mg/dl. Its physiological roles are numerous and varied, but with several functions similar to those of immunoglobulins, CRP appears to function in host defense.\n\nCRP is one of the acute-phase proteins, the serum or plasma levels of which rise during general, nonspecific response to a wide variety of diseases. This include infections by gram-positive and gram-negative organisms, acute phase of rheumatoid arthritis, abdominal abscesses, and inflammation of the bile duct. CRP may also be found in patients with Guillain-Barre syndrome and multiple sclerosis, certain viral infections, tuberculosis, acute infectious hepatitis, many other necrotic and inflammatory diseases, burned patients and after surgical trauma. \n\nAlthough the detection of elevated levels of CRP in the serum is not specific for any particular disease, it is a useful indicator of inflammatory processes. CRP levels rise in serum or plasma within 24 to 48 hours following acute tissue damage, reach a peak during the acute stage (approximately 1000x constitutive level) and decrease with the resolution of inflammation or trauma. The concentration increase of CRP in human serum or plasma may last for several days before decreasing to normal levels. \n\nThe detection of CRP is a more reliable and sensitive indicator of the inflammatory process than the erythrocyte sedimentation rate13, which may also be influenced by physiological changes not associated with an inflammatory process. Current testing methods including latex agglutination, nephelometry, and radial immunodiffusion (RID) have the general disadvantages of low sensitivity, whereas ELISAs provide the highest sensitivity and specificity. \n\nAs elevated CRP values are always associated with pathological changes, the CRP assay provides useful information for the research of inflammatory processes and associated disease. Additionally, measurement of CRP by high-sensitivity CRP assays may add to the predictive value of other cardiac markers (myoglobin, creatine-kinase-MB, troponin I and T), which are used to assess the risk of cardiovascular and peripheral vascular disease. \n\nDetection Range:\n0-0.1ug/ml\n\nSample Volume:\n5ul\n\nSpecificity:\n96%\n\nSensitivity:\n0.01ug/ml\n\nTotal Time:\n~65 minutes\n\nKit Components:\nC7907-02P1: Microtiter Plate, 1x96 wells\nC7907-02P2: Standard, 0ug/ml, 1x1ml\nC7907-02P3: Standard 0.005ug/ml, 1x1ml\nC7907-02P4: Standard 0.01ug/ml, 1x1ml\nC7907-02P5: Standard 0.025ug/ml, 1x1ml\nC7907-02P6: Standard 0.05ug/ml, 1x1ml\nC7907-02P7: Standard 0.1ug/ml, 1x1ml\nC7907-02P8: Sample Diluent, 1x50ml\nC7907-02P9: Pab (HRP) Reagent, 1x12ml\nC7907-02P10: TMB Solution, 1x11ml\nC7907-02P11: Stop Solution, 1N HCl, 1x11ml\n \nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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