C57BL/6 Mouse Bone Marrow Endothelial Progenitor Cells are derived from the tibias and femurs of pathogen-free laboratory adult mice. C57BL/6 Mouse Bone Marrow Endothelial Progenitor Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Creative Bioarray's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.
Standard biochemical procedures performed with progenitor cell cultures include cell adhesion, migration, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.