CA-12-5 BioAssay(TM) ELISA Kit

The CA-125 Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The assay system utilizes one monoclonal anti-CA-125 antibody for solid phase (microtiter wells) immobilization and another monoclonal anti-CA 125 antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The standards and test specimen (serum) are added to the CA-125 antibody coated microtiter wells. Then CA-125 antibody labeled with horseradish peroxidase (conjugate) is added. If human CA-125 is present in the specimen, it will combine with the antibody on the well and the enzyme conjugate resulting in the CA-125 molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 3 hour incubation at 37 degrees C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of 2N HCl. The color is changed to yellow and measured spectrophotometrically at 450 nm. The concentration of CA-125 is directly proportional to the color intensity of the test sample.\n\nIntended Use:\nCA-125 EIA test is primarily intended for use as a monitoring and screening test. An abnormal result (i.e., elevated serum CA-125 level) can indicate ovarian cancer and suggests the need for further clinical management. The serum CA-125 test appears to be a useful tumor marker for patients in clinical remission, following treatment. Post-operative serum CA-125 values that fail to return to normal, strongly suggest the presence of residual tumor. Tumor recurrence is often accompanied by a rise of serum CA-125 before progressive disease is clinically evident.\n\nDetection Range: 0-400U/ml\n\nSpecificity: 97%\n\nSensitivity: 5units/ml\n\nKit Components: \nMicrotiter Plate, 1x96wells\nEnzyme Conjugate Reagent, 1x12ml\nStandard 0units/ml, 1x1vial\nStandard 15units/ml, 15units/ml, 1x1vial\nStandard 15units/ml, 50units/ml, 1x1vial\nStandard 15units/ml, 100units/ml, 1x1vial\nStandard 15units/ml, 200units/ml, 1x1vial\nStandard 15units/ml, 400units/ml, 1x1vial\nTMB Substrate, 1x12ml\nStop Solution, 1x12ml\nWash Buffer Concentrate (50X), 1x15ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167546

Size

96Tests

Applications

ELISA

References

1. Kenemans P, Yedema CA, Bon GG, von Mensdorff-Pouilly S. Ca125 in gynecological pathology a review. Eur J Obstet Gynecol 1993;49:115-124.\n2. Saksela F. Prognostic markers in epithelial ovarian cancer. Intl J Gynecol Pathol 1993;12:156-161. 3. Farghaly SA. Tumor markers in gynecologic cancer. Gynecol & Obstet Invest 1992;34:65-72. 4. Welander CE. What do CA 125 and other antigens tell us about ovarian cancer biology. Acta Obstet Gynecol Scand Sup 1992;155:85-93. 5. McGowan L. Pathology of the ovary. Curr Opin on Obstet Gynecol 1991;3:580-586. 6. Niloff JM. Ovarian malignancy. Curr Opin on Obstet Gynecol 1991;3:66-72. 7. Olt G, Berchuck A, Bast RC. The role of tumor markers in gynecologic oncology. Obstet Gynecol Survey 1990;45-:570-577. 8. Diez M, Cerdan FJ, Ortega MD, Torres A, Picardo A, Balibrea JL. Evaluation of serum CA-125 as a tumor\nmarker in non-small cell lung cancer. Cancer 1991;67:150-154. 9. Niloff JM, Klug TL, Schaetzl E. Elevation of serum CA-125 in carcinomas of the fallopian tube, endometrium, and endocervix. AM J. Obstet Gynecol 1984; 148:1057.