CA19-9 (Sialyl Lewis a) is a carbohydrate antigenic determinant identified as a sialylated lacto-N-fucopentose II, an oligosaccharide biochemically related to the Lewis a blood group antigen. In malignant tissues positive staining of tumor cells is observed in most adenocarcinomas of the pancreas and stomach, and in a large proportion of colon and gall bladder tumors. The antigen is also expressed in approximately half of primary and metastatic ovarian tumors. In normal tissues, positive staining is also observed in column or epithelium of the pancreas stomach, liver, gall bladder and in bronchial glands of the lung.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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