Cadherin 11, Recombinant, Mouse, Fc Chimera (Cadherin-11, OSF-4, Osteoblast Cadherin, OB-cadherin, Cdh11, Cad-11)

Cadherin-11, also known as OB-Cadherin, is a 120kD member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity. Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems. It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes. Cadherin-11 is up-regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone. It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11. In the synovium, Cadherin-11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis. Its up-regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation. In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants. Mature mouse Cadherin-11 consists of a 564aa extracellular domain (ECD) with five tandem Cadherin repeats, a 23aa transmembrane segment, and a 156aa cytoplasmic domain. Within the ECD, mouse Cadherin-11 shares 97% and 98% aa sequence identity with human and rat Cadherin-11, respectively. An 80kD portion of the Cadherin-11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion.\n\nSource:\nRecombinant corresponding to aa1-617 from mouse Cadherin-11 Fc Chimera expressed in mouse myeloma cell line, NS0.\n\nMolecular Weight:\n~89.1kD (mature protein, monomer), 92.8kD (pro-protein, monomer)\n\nBiological Activity:\nMeasured by the ability of the immobilized protein to support the adhesion of CCRT cotton rat osteosarcoma cells. The ED50 for this effect is typically 0.35-1.4ug/ml.\n\nEndotoxin: ~1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer . Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0107-06M

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with PBS to 0.1mg/ml.

Purity

~90% (SDS-PAGE)

References

1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.2. Kimura, Y. et al. (1995) Dev. Biol. 169:347. 3. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337. 4. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78. 5.Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909. 6. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840. 7. Tamura, D. et al. (2008) Int. J. Oncol. 33:17. 8. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259. 9. Huang, C.