Cadherins are a family of transmembrane glycoproteins that play a key role in Calcium dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal) and M- (muscle) cadherin. M-Cadherin is expressed in myoblasts and might be involved in skeletal muscle cell differentiation.
Applications:
Suitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 5ug/ml
ELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT (MTT) detection; 0.5ug/ml for HRP/ECL detection
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.