CALB1, Recombinant, Human (Calbindin, Calbindin D28, D-28K, Vitamin D-dependent Calcium-binding Protein, Avian-type, CAB27)

Calcium binding proteins (CBPs) regulate intracellular levels of calcium (Ca2+) ions. CBPs are involved in numerous functions, such as cell signaling, calcium uptake and transport, and cell motility. Calretinin (CR), calbindin D-28k (CB) and parvalbumin (PV) belong to the large family of EF-hand calcium-binding proteins. The EF-hand is an aa sequence with a characteristic three-dimensional helix-loop-helix structure with high affinity for Ca2+. Calbindin D28k (calbindin) is a hexa EF-hand protein of 261 residues with 59% sequence identity to calretinin. It is a single-domain protein and the six EF hands interact extensively with one another so that they form one large globular domain. Calbindin is found in the brain and central nervous system with a distinct distribution over certain neuronal subtypes. Neuroprotective effects of calbindin has been reported in ischemic and glutamate toxicity models, primarily due to its ability to chelate calcium. Mice lacking calbindin have been shown to suffer from impaired motor coordination due to altered neuronal Ca2+-homeostasis. Notably, calbindin is found in high concentration in the kidney and in pancreatic b-cells, where it has been shown to protect against apoptosis. In osteoblasts, anti-apoptic activity of calbindin through the binding to caspase-3 has also been reported. Calbindin protects against apoptotic and necrotic cell death, suggesting its ability to buffer calcium. Calbindin has been reported to binds Zn2+, revealing negative allosteric effect between the Zn2+- and Ca2+-binding events.\n\nSource:\nRecombinant corresponding to aa1-261, with an additional 3aa-GSH at N-terminal, from human Calbinind, expressed in E.coli.\n\nMolecular Weight:\n~30kD\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C0113-14B

Size

200ug

Form

Supplied as a lyophilized powder from 20mM Tris, pH 8, 10mM sodium chloride. Reconstitute with sterile dH2O PBS.

Purity

~95% (SDS-PAGE)

References

1. Bauer MC et al. 2008, Protein Sci. 17(4):760-767. 2. Anelli R and Heckman CJ, 2005, J Neurocytol. 34(6):369-385. 3. Linse S et al. 1997, Protein Sci. 6(11):2385-2396.