The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization.
Application:
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.