Calbindin D-9K, Recombinant, Rat (D9K, CABP9K, CALB3, CABP1)

CABP9K (CALB3 or CABP1; mouse, rat, and human 79 aa; chromosome Xp; ~9 kD) is a cytosolic Ca-binding protein initially found in rat pancreas. It is also expressed in intestine, placenta, uterus and kidney. Its expression is controlled by vitamin D and sex hormone in a tissue specific manner. In keeping with its role in Ca-transport, its expression is highest in duodenal villus enterocytes. It is further shown that CABP9K is only expressed in differentiated enterocytes. CABP9K gene also contains Cdx2-homeoprotein binding sites, and that Cdx2 may play a crucial role in CABP9K transcription. CaBP9K is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized CABP9K in sterile 18MOmega-cm HO not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized CABP9K although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution CABP9K should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

C0113-20

Size

1ug

Form

Supplied as a lyophilized powder in 100mM phosphate buffer, pH 7.3.

Purity

(same/more than) 90% as determined by reducing and non-reducing SDS-PAGE Coomassie.

References

1. Anti-progestogenic effect of flutamide on uterine expression of calbindin-D(9k) mRNA and protein in immature mice.\n\nReprod Toxicol 2006 Jun 13;\n\n2. Assignment strategy for fast relaxing signals: complete aminoacid identification in thulium substituted calbindin D 9K.\n\nJ Biomol NMR 2006 Feb;34(2):63-73\n\n3. Complex regulation of Calbindin-D(9k) in the mouse placenta and extra-embryonic membrane during mid- and late pregnancy.\n\nMol Cell Endocrinol 2004 Feb 12;214(1-2):39-52\n\n4. Effect of age and dietary calcium on intestinal calbindin D-9k expression in the rat.\n\nArch Biochem Biophys 2003 Dec 1;420(1):194-200\n\n5. Mouse calbindin-D(9k) gene expression in the uterus during late pregnancy and lactation.\n\nMol Cell Endocrinol 2003 Jul 31;205(1-2):79-88\n\n6. Expression of human Calbindin-D(9k) correlated with age, vitamin D receptor and blood calcium level in the gastrointestinal tissues.\n\nClin Biochem 2003 Jun;36(4):255-61