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Calbindin (D28K, Vitamin D-dependent Calcium-binding Protein, CALB1)

Calbindin (D28K, Vitamin D-dependent Calcium-binding Protein, CALB1)

Cat no: C0113-16A

Supplier: United States Biological
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Calbindin-D is a highly conserved 28 kD (261 aa) protein that belongs to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin-D has 4 active calcium binding domains. It plays a role in the regulation of calcium and calcium related signalling in neurons and during embryo development. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1.0ug/ml, has a predicted length of 261aa and a MW of 28kD Immunohistochemistry (formalin fixed and paraffin embedded tissues) 1-2ug/ml Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C0113-16A
Reactivities: Rat, Bovine
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
P type: Mab
Isotype: IgG
Purity: Purified by affinity chromatography.
Alternative names: CALB1_BOVIN, CALB1
Additional info: Recognizes rat Calbindin. Reacs only with calbindin-D-28K and not with other family members such as calretinin and calbindin-D-9K. Species sequence homology: bovine.

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