Calcineurin is a major calmodulin binding protein in the brain. It is a holoenzyme composed of a calmodulin binding subunit, a catalytic alpha subunit (calcineurin A) and a calcium binding beta subunit (calcineurin B). The calcineurin A subunit has at least two isoforms in bovine brain; alpha 1 (61kD) and alpha 2 (59kD)
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 0.25-0.50, has a predicted length of 521aa and a MW of 59kD
Immunohistochemistry (formalin fixed and paraffin embedded tissues) 1-2ug/ml
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.