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Calcineurin alpha (CNA, CALNA, PPP3CA)

Cat no: C0114-06C


Supplier: United States Biological
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Calcineurin is a major calmodulin binding protein in the brain. It is a holoenzyme composed of a calmodulin binding subunit, a catalytic alpha subunit (calcineurin A) and a calcium binding beta subunit (calcineurin B). The calcineurin A subunit has at least two isoforms in bovine brain; alpha 1 (61kD) and alpha 2 (59kD) Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25-0.50, has a predicted length of 521aa and a MW of 59kD Immunohistochemistry (formalin fixed and paraffin embedded tissues) 1-2ug/ml Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C0114-06C
Reactivities: Rat, Bovine
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.2% sodium azide. Reconstitute with sterile PBS to concentration of 0.1-1mg/ml.
P type: Mab
Isotype: IgG
Purity: Purified by affinity chromatography.
Alternative names: CNA, CALNA, PPP3CA,
Additional info: Recognizes rat Calcineurin alpha. Reacts only with the isoforms of the calcineurin A subunit. Species sequence homology: bovine.

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